Chimeric regulatory light chains as probes of smooth muscle myosin function.
نویسندگان
چکیده
منابع مشابه
Structural requirement of the regulatory light chain of smooth muscle myosin as a substrate for myosin light chain kinase.
The substrate structure required for skeletal and smooth muscle myosin light chain kinases (MLC kinase) was studied by using various mutant regulatory light chains of smooth muscle myosin. The deletion of the NH2-terminal 10 residues did not greatly affect the kinetic parameters of smooth MLC kinase; however, deletion of an additional 3 residues, Lys11-Arg13, prevented phosphorylation. In contr...
متن کاملRoles of light chains in the activity and conformation of smooth muscle myosin.
The 20-kDa regulatory (LC20) and 17-kDa essential (LC17) light chain subunits could be removed from porcine aorta smooth muscle myosin by the use of trifluoperazine and ammonium chloride. The isolated heavy chain rebound both light chains, resulting in the restoration of native properties. Experiments on reconstitution of the isolated heavy chain with LC17 and/or LC20 showed that both light cha...
متن کاملPhosphorylation of smooth muscle myosin heavy and light chains. Effects of phorbol dibutyrate and agonists.
A number of different protein kinases phosphorylate purified heavy chains or the 20-kDa light chain of smooth muscle myosin. The physiological significance of these phosphorylation reactions has been examined in intact smooth muscle. Myosin heavy chain was slightly phosphorylated (0.08 mol of phosphate/mol) under control conditions in bovine tracheal tissue. Treatment with carbachol, isoprotere...
متن کاملMyosin light-chain kinase of smooth muscle stimulates myosin ATPase activity without phosphorylating myosin light chain.
Myosin light-chain kinase (MLCK) of smooth muscle is multifunctional, being composed of N-terminal actin-binding domain, central kinase domain, and C-terminal myosin-binding domain. The kinase domain is the best characterized; this domain activates the interaction of smooth-muscle myosin with actin by phosphorylating the myosin light chain. We have recently shown that the Met-1-Pro-41 sequence ...
متن کاملPhosphorylation of the regulatory light chains of myosin affects Ca sensitivity of skeletal muscle contraction
Szczesna, Danuta, Jiaju Zhao, Michelle Jones, Gang Zhi, James Stull, and James D. Potter. Phosphorylation of the regulatory light chains of myosin affects Ca2 sensitivity of skeletal muscle contraction. J Appl Physiol 92: 1661–1670, 2002. First published December 21, 2001; 10.1152/japplphysiol.00858.2001.—The role of phosphorylation of the myosin regulatory light chains (RLC) is well establishe...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1993
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)53625-5